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We now take an aliquot (10 ul or 20 ul) out, dry the alcohol, then add 200 ul mixture of JH antibody and radio-labeled JH. JH in the sample will compete with labeled JH to bind to the antibody. Because binding sites are limited, the more JH we have in a sample, the more radiolabeled JH will be displaced from the antibody. Incubation is carried out for 2 hours at room temperature.


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